Journal: Trends in Hearing

Article Title: Harmonic Frequency Lowering

doi: 10.1177/2331216515626131

Figure Lengend Snippet: Characteristics of the Test Participants and the Individual Parameters for the Nonlinear Frequency Compression Algorithm: Audiometric Data (dB HL), Age (yrs), Hearing Aid Experience/HAE (yrs), and Music Experience/ME (Scale min: −3, Scale max: 4), Cut-off Frequency/Fc, and Compression Ratio/CR for the NFC (Nonlinear Frequency Compression) Condition.

Article Snippet: P14 , 20 , 30 , 35 , 35 , 50 , 60 , 70 , 65 , 30 , 30 , 35 , 40 , 40 , 65 , 85 , 80 , M , 69 , Oticon Agil , 9 , −2.5 , 4.5 , 2.7.

Techniques:

Journal: Journal of proteomics

Article Title: Development of an AlphaLISA assay to quantify serum core-fucosylated E-cadherin as a metastatic lung adenocarcinoma biomarker.

doi: 10.1016/j.jprot.2012.05.015

Figure Lengend Snippet: Fig. 6 – AlphaLISA assay. (A) Effect of biotin-AAL concentration on the AlphaLISA signal for the detection of fucosylated E-cadherin. Different volumes of serum were preincubated with different concentrations of biotinylated-AAL and anti-E-cadherin antibody-protein A acceptor beads for 60 min at room temperature before addition of streptavidin donor beads and incubation for a further 60 min at room temperature before reading the plate. (B) Effect of biotin–anti-E-cadherin monoclonal antibody concentration on the AlphaLISA signal for detection of total E-cadherin. Different volumes of serum were preincubated with different concentrations of biotin-anti-E-cadherin monoclonal antibody and rabbit anti-E-cadherin polyclonal antibody-anti-rabbit IgG-conjugated acceptor beads for 60 min at room temperature before addition of streptavidin donor beads and incubation for a further 60 min at room temperature before reading the plate. (C) The concentrations of 1 μM biotin-AAL and 10 μM biotin-α-E-cadherin monoclonal antibody were chosen for FI evaluation using a 1:50 serum dilution. The fucosylated index of E-cadherin in serum samples from 30 patients with early or late stage lung adenocarcinoma and controls measured by AlphaLISA.

Article Snippet: The AlphaLISA was performed in 96 well microtiter plates (white opaque OptiPlateTM from Perkin Elmer) containing 5 μL of diluted serum, 10 μL of biotinylated E-cadherin monoclonal antibody, and 10 μL of rabbit anti-E-cadherin polyclonal antibodies conjugated to anti-rabbit IgG-conjugated acceptor beads, all in AlphaLISA buffer (30 mM Tris, pH 7.4, 0.1% Tween 20, 0.1% casein, and 1mg/mL dextran T500).

Techniques: Concentration Assay, Incubation

Journal: Journal of proteomics

Article Title: Development of an AlphaLISA assay to quantify serum core-fucosylated E-cadherin as a metastatic lung adenocarcinoma biomarker.

doi: 10.1016/j.jprot.2012.05.015

Figure Lengend Snippet: Fig. 7 – Comparison of the FI for E-cadherin in lung cancer sera using the AlphaLISA method or the Western blot and lectin staining method.

Article Snippet: The AlphaLISA was performed in 96 well microtiter plates (white opaque OptiPlateTM from Perkin Elmer) containing 5 μL of diluted serum, 10 μL of biotinylated E-cadherin monoclonal antibody, and 10 μL of rabbit anti-E-cadherin polyclonal antibodies conjugated to anti-rabbit IgG-conjugated acceptor beads, all in AlphaLISA buffer (30 mM Tris, pH 7.4, 0.1% Tween 20, 0.1% casein, and 1mg/mL dextran T500).

Techniques: Comparison, Western Blot, Staining